Journal: Animals : an Open Access Journal from MDPI

Article Title: Early Pregnancy Induces Expression of STAT1, OAS1 and CXCL10 in Ovine Spleen

doi: 10.3390/ani9110882

Figure Lengend Snippet: Primers used for RT-qPCR.

Article Snippet: The membranes were incubated with a goat anti-STAT1 polyclonal antibody (Abcam, Cambridge, UK, ab230428, 1:1000), a rabbit anti-OAS1 polyclonal antibody (Abcam, ab86343, 1:1000), a mouse anti-Mx1 monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-166412, 1:1000), and a mouse anti-CXCL10 monoclonal antibody (Santa Cruz Biotechnology, sc-374092, 1:1000), respectively.

Techniques: Sequencing

Journal: Animals : an Open Access Journal from MDPI

Article Title: Early Pregnancy Induces Expression of STAT1, OAS1 and CXCL10 in Ovine Spleen

doi: 10.3390/ani9110882

Figure Lengend Snippet: Relative expression values of STAT1 , OAS1 , MX1 and CXCL10 mRNA in ovine spleens measured by real-time quantitative PCR. Note: DN16 = Day 16 of the estrous cycle; DP13 = Day 13 of pregnancy; DP16 = Day 16 of pregnancy; DP25 = Day 25 of pregnancy. Significant differences ( p < 0.05) are indicated by different letters within same color column.

Article Snippet: The membranes were incubated with a goat anti-STAT1 polyclonal antibody (Abcam, Cambridge, UK, ab230428, 1:1000), a rabbit anti-OAS1 polyclonal antibody (Abcam, ab86343, 1:1000), a mouse anti-Mx1 monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-166412, 1:1000), and a mouse anti-CXCL10 monoclonal antibody (Santa Cruz Biotechnology, sc-374092, 1:1000), respectively.

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: Animals : an Open Access Journal from MDPI

Article Title: Early Pregnancy Induces Expression of STAT1, OAS1 and CXCL10 in Ovine Spleen

doi: 10.3390/ani9110882

Figure Lengend Snippet: Expression of STAT1, OAS1, Mx1 and CXCL10 proteins in ovine spleens analyzed by western blot. Note: DN16 = Day 16 of the estrous cycle; DP13 = Day 13 of pregnancy; DP16 = Day 16 of pregnancy; DP25 = Day 25 of pregnancy. Significant differences ( p < 0.05) are indicated by different superscript letters within the same color column.

Article Snippet: The membranes were incubated with a goat anti-STAT1 polyclonal antibody (Abcam, Cambridge, UK, ab230428, 1:1000), a rabbit anti-OAS1 polyclonal antibody (Abcam, ab86343, 1:1000), a mouse anti-Mx1 monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-166412, 1:1000), and a mouse anti-CXCL10 monoclonal antibody (Santa Cruz Biotechnology, sc-374092, 1:1000), respectively.

Techniques: Expressing, Western Blot

Journal: Vaccines

Article Title: Therapeutic Anti-Tumor Efficacy of DC-Based Vaccines Targeting TME-Associated Antigens Is Improved When Combined with a Chemokine-Modulating Regimen and/or Anti-PD-L1

doi: 10.3390/vaccines12070777

Figure Lengend Snippet: Therapeutic DC–Pool Peptide vaccines promote reduced vascular content and coordinate increased levels of CD8 + T cells and CD11c + DC and increased expression of CXCL10 in the B16 TME. Day 24 tumors harvested from DC–Pool Peptide vaccine cohorts described in were analyzed by immunofluorescence microscopy for their content of CD8 + TIL ( A,B ), CD11c + DC ( A,C ), CD31 + vessels ( D,E ), and CXCL10 ( D,F ). Experimental details are provided in Materials and Methods ( n = 8 mice/group). * p < 005, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 (Student’s t test). ( E , F ).

Article Snippet: Fluorescently labeled antibodies used to stain tumor tissue sections included the following: Alexa 488-conjugated rabbit anti-mouse CD8α (Abcam), Alexa 594-conjugated hamster anti-mouse CD11c (Biolegend), Alexa 488-conjugated rabbit anti-mouse CD31 (Abcam), and PE-conjugated rat anti-mouse CXCL10 (R&D Systems).

Techniques: Vaccines, Expressing, Immunofluorescence, Microscopy

Journal: Arthritis Research & Therapy

Article Title: Coexpression and interaction of CXCL10 and CD26 in mesenchymal cells by synergising inflammatory cytokines: CXCL8 and CXCL10 are discriminative markers for autoimmune arthropathies

doi: 10.1186/ar1997

Figure Lengend Snippet: CXCL8 and CXCL10 induction in fibroblasts by IL-1β and interferons . Confluent fibroblast monolayers were incubated with IL-1β in combination with IFN-α, IFN-β or IFN-γ. Results represent the mean CXCL8 and CXCL10 protein concentration (ng/ml) measured in the culture supernatant (three or more independent experiments).

Article Snippet: The sandwich ELISA for human CXCL10 consisted of mouse monoclonal anti-human CXCL10 (R&D Systems) as a coating antibody, biotinylated rabbit polyclonal anti-human CXCL10 (R&D Systems) as a capturing antibody and peroxidase-conjugated streptavidin (Jackson ImmunoResearch Laboratories) as a detecting antibody.

Techniques: Incubation, Protein Concentration

Journal: Arthritis Research & Therapy

Article Title: Coexpression and interaction of CXCL10 and CD26 in mesenchymal cells by synergising inflammatory cytokines: CXCL8 and CXCL10 are discriminative markers for autoimmune arthropathies

doi: 10.1186/ar1997

Figure Lengend Snippet: CXCL8 and CXCL10 induction in fibroblasts by tumour necrosis factor alpha and interferons . Confluent fibroblast monolayers were incubated with tumour necrosis factor alpha (TNF-α) in combination with IFN-α, IFN-β or IFN-γ. Results represent the mean CXCL8 and CXCL10 concentration (ng/ml) measured in the culture supernatant (three or more independent experiments).

Article Snippet: The sandwich ELISA for human CXCL10 consisted of mouse monoclonal anti-human CXCL10 (R&D Systems) as a coating antibody, biotinylated rabbit polyclonal anti-human CXCL10 (R&D Systems) as a capturing antibody and peroxidase-conjugated streptavidin (Jackson ImmunoResearch Laboratories) as a detecting antibody.

Techniques: Incubation, Concentration Assay

Journal: Arthritis Research & Therapy

Article Title: Coexpression and interaction of CXCL10 and CD26 in mesenchymal cells by synergising inflammatory cytokines: CXCL8 and CXCL10 are discriminative markers for autoimmune arthropathies

doi: 10.1186/ar1997

Figure Lengend Snippet: CXCL10 induction by combinations of interferons in fibroblasts and human microvascular endothelial cells . Monolayers of fibroblasts or human microvascular endothelial cells (HMVEC) were incubated with combinations of IFN-α or IFN-β and IFN-γ. Results represent the mean CXCL10 concentration (ng/ml) measured in the culture supernatant (three or more independent experiments).

Article Snippet: The sandwich ELISA for human CXCL10 consisted of mouse monoclonal anti-human CXCL10 (R&D Systems) as a coating antibody, biotinylated rabbit polyclonal anti-human CXCL10 (R&D Systems) as a capturing antibody and peroxidase-conjugated streptavidin (Jackson ImmunoResearch Laboratories) as a detecting antibody.

Techniques: Incubation, Concentration Assay

Journal: Arthritis Research & Therapy

Article Title: Coexpression and interaction of CXCL10 and CD26 in mesenchymal cells by synergising inflammatory cytokines: CXCL8 and CXCL10 are discriminative markers for autoimmune arthropathies

doi: 10.1186/ar1997

Figure Lengend Snippet: CXCL8 and CXCL10 induction in human microvascular endothelial cells by IL-1β and interferons . Human microvascular endothelial cells (HMVEC) were incubated with IL-1β in combination with IFN-α, IFN-β or IFN-γ. Results represent the mean CXCL8 and CXCL10 concentration (ng/ml) measured in the culture supernatant (three or more independent experiments).

Article Snippet: The sandwich ELISA for human CXCL10 consisted of mouse monoclonal anti-human CXCL10 (R&D Systems) as a coating antibody, biotinylated rabbit polyclonal anti-human CXCL10 (R&D Systems) as a capturing antibody and peroxidase-conjugated streptavidin (Jackson ImmunoResearch Laboratories) as a detecting antibody.

Techniques: Incubation, Concentration Assay

Journal: Arthritis Research & Therapy

Article Title: Coexpression and interaction of CXCL10 and CD26 in mesenchymal cells by synergising inflammatory cytokines: CXCL8 and CXCL10 are discriminative markers for autoimmune arthropathies

doi: 10.1186/ar1997

Figure Lengend Snippet: CXCL8 and CXCL10 induction in HMVEC by tumour necrosis factor alpha and interferons . Human microvascular endothelial cells (HMVEC) were incubated with tumour necrosis factor alpha (TNF-α) in combination with IFN-α, IFN-β or IFN-γ. Results represent the mean CXCL8 and CXCL10 concentration (ng/ml) measured in the culture supernatant (three or more independent experiments).

Article Snippet: The sandwich ELISA for human CXCL10 consisted of mouse monoclonal anti-human CXCL10 (R&D Systems) as a coating antibody, biotinylated rabbit polyclonal anti-human CXCL10 (R&D Systems) as a capturing antibody and peroxidase-conjugated streptavidin (Jackson ImmunoResearch Laboratories) as a detecting antibody.

Techniques: Incubation, Concentration Assay

Journal: Arthritis Research & Therapy

Article Title: Coexpression and interaction of CXCL10 and CD26 in mesenchymal cells by synergising inflammatory cytokines: CXCL8 and CXCL10 are discriminative markers for autoimmune arthropathies

doi: 10.1186/ar1997

Figure Lengend Snippet: Reverse-phase HPLC purification of fibroblast-derived CXCL10 . Semi-purified fibroblast-derived CXCL10 was subjected to C8 reverse-phase HPLC. Proteins were eluted in an acetonitrile gradient (dashed line) and UV absorbance was detected at 214 nm (solid line). CXCL10 immunoreactivity in the column fractions was detected by ELISA (histograms).

Article Snippet: The sandwich ELISA for human CXCL10 consisted of mouse monoclonal anti-human CXCL10 (R&D Systems) as a coating antibody, biotinylated rabbit polyclonal anti-human CXCL10 (R&D Systems) as a capturing antibody and peroxidase-conjugated streptavidin (Jackson ImmunoResearch Laboratories) as a detecting antibody.

Techniques: Purification, Derivative Assay, Enzyme-linked Immunosorbent Assay

Journal: Arthritis Research & Therapy

Article Title: Coexpression and interaction of CXCL10 and CD26 in mesenchymal cells by synergising inflammatory cytokines: CXCL8 and CXCL10 are discriminative markers for autoimmune arthropathies

doi: 10.1186/ar1997

Figure Lengend Snippet: Identification of fibroblast-derived CXCL10 . The relative molecular mass ( M r ) of reverse-phase-HPLC-purified CXCL10 was determined by electrospray ion trap mass spectrometry. Results show the (a) averaged and (b) averaged deconvoluted spectra of CXCL10 that eluted in between 26% and 28% acetonitrile from the C8 column (Figure 6). The amino acids cleaved off (one-letter code), explaining the differences between the CXCL10 isoforms, are indicated on top of the averaged deconvoluted spectrum. Both NH 2 -terminally truncated, COOH-terminally intact CXCL10(3–77), CXCL10(4–77), CXCL10(5–77), CXCL10(6–77), and NH 2 -terminally and COOH-terminally cleaved CXCL10(3–73), CXCL10(4–73), CXCL10(5–73) and CXCL10(6–73) were identified. The deviation between the theoretical and the experimentally determined average M r for each amino acid is indicated below the one-letter code.

Article Snippet: The sandwich ELISA for human CXCL10 consisted of mouse monoclonal anti-human CXCL10 (R&D Systems) as a coating antibody, biotinylated rabbit polyclonal anti-human CXCL10 (R&D Systems) as a capturing antibody and peroxidase-conjugated streptavidin (Jackson ImmunoResearch Laboratories) as a detecting antibody.

Techniques: Derivative Assay, Purification, Mass Spectrometry

Journal: Arthritis Research & Therapy

Article Title: Coexpression and interaction of CXCL10 and CD26 in mesenchymal cells by synergising inflammatory cytokines: CXCL8 and CXCL10 are discriminative markers for autoimmune arthropathies

doi: 10.1186/ar1997

Figure Lengend Snippet: CXCR3-dependent signalling . Serum-starved Chinese hamster ovary CXCR3 cells were treated with Ham's F-12 medium supplemented with 0.5% foetal bovine serum (FBS) or stimulated with CXCL10 or NH 2 -terminally truncated CXCL10(3–77) at a concentration of 1, 10 or 100 ng/ml (in Ham's F-12 supplemented with 0.5% FBS). The reaction was stopped after 5 minutes and the cells were lysed. The level of extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation or protein kinase B/Akt phosphorylation in the cell lysate was determined with specific ELISAs for phosphoERK or phosphoAkt. The mean values ( n = 4) and standard errors are indicated. *Statistically significant differences (Mann–Whitney U test) from control ( P < 0.05).

Article Snippet: The sandwich ELISA for human CXCL10 consisted of mouse monoclonal anti-human CXCL10 (R&D Systems) as a coating antibody, biotinylated rabbit polyclonal anti-human CXCL10 (R&D Systems) as a capturing antibody and peroxidase-conjugated streptavidin (Jackson ImmunoResearch Laboratories) as a detecting antibody.

Techniques: Concentration Assay, Phospho-proteomics, MANN-WHITNEY, Control

Journal: Arthritis Research & Therapy

Article Title: Coexpression and interaction of CXCL10 and CD26 in mesenchymal cells by synergising inflammatory cytokines: CXCL8 and CXCL10 are discriminative markers for autoimmune arthropathies

doi: 10.1186/ar1997

Figure Lengend Snippet: CXCL8 and CXCL10 in synovial fluid of arthritis patients . (a) CXCL10 and (b) CXCL8 concentrations were measured by ELISAs in synovial fluids of patients with ankylosing spondylitis (AS), psoriatic arthritis (PsA) and rheumatoid arthritis (RA), and were compared with chemokine concentrations in the metabolic arthritis patients with crystal-induced arthritis (CA). The detection limits of the ELISAs for the synovial concentrations of CXCL8 and CXCL10 are indicated on the y axis (logarithmic scale) and were 0.25 ng/ml and 1 ng/ml, respectively. Statistical analysis was performed with the median levels (dashed bars) using the nonparametric Mann–Whitney U test.

Article Snippet: The sandwich ELISA for human CXCL10 consisted of mouse monoclonal anti-human CXCL10 (R&D Systems) as a coating antibody, biotinylated rabbit polyclonal anti-human CXCL10 (R&D Systems) as a capturing antibody and peroxidase-conjugated streptavidin (Jackson ImmunoResearch Laboratories) as a detecting antibody.

Techniques: MANN-WHITNEY